Laboratory Standard Operating Procedures (SOPs) were developed to ensure the safety and proper operation of all equipment and research procedures at the Ragon Institute. The following documents describe the best practices for regularly recurring operations relevant to the quality of the investigation. It is the responsibility of all to familiarize themselves with these procedures and follow them correctly.
Ragon Institute Operational SOPs (RIO-SOP)
SOP Number | Title | Date | Download Link |
RIO-SOP-001 | Ragon Alarm Instructions | 7/4/2017 | RIO_SOP_001 |
RIO-SOP-002 | Alarm Pager Instructions | 7/3/2017 | RIO_SOP_002 |
RIO-SOP-003 | Ultracentrifuge Operation at Ragon Institute | 11/23/2017 | RIO_SOP_003 |
RIO-SOP-004 | X-RAD Operation Instructions | 11/23/2017 | RIO_SOP_004 |
RIO-SOP-005 | Autoclave Operation | 1/21/2019 | RIO_SOP_005 |
RIO-SOP-006 | Cage Space Allocation | 12/6/2022 | RIO_SOP_006 |
Laboratory Safety (LS-SOP)
SOP Number | Title | Date | Download Link |
LS-SOP-001 | Ragon Laboratory Dress Code | 4/25/2017 | LS_SOP_001 |
LS-SOP-002 | Emergency Procedure for Accidental Exposure | 4/24/2017 | LS_SOP_002 |
LS-SOP-003 | General Rules and Safety Requirements in the BSL2+ laboratory areas | 01/08/2019 | LS_SOP_003 |
LS-SOP-004 | Principles of Biosafety Training in the BSL2+ Facility at Ragon Insitute | 6/28/2017 | LS_SOP_004 |
LS-SOP-005 | General Rules and Safety requirements in the Biosafety Level 2 (BSL2) laboratory areas | 01/08/2019 | LS_SOP_005 |
LS-SOP-006 | General Rules and Safety requirements in the Biosafety Level 2 (BSL2) laboratory areas when working with dengue virus serotypes 1-4 | 01/08/2018 | LS_SOP_006 |
LS-SOP-007 | Biohazardous Waste Disposal Procedure | 06/07/2019 | LS_SOP_007 |
LS-SOP-008 | Ragon Institute BSL2+ Tissue Culture Waste Disposal Guidelines | 12/4/2019 | LS_SOP_008 |
Shipment and Transport (ST-SOP)
SOP number | Title | Date | Download Link |
ST-SOP-001 | Sample and Specimen Transfer Protocol | 6/24/2017 | ST_SOP_001 |
ST-SOP-002 | World Courier International Shipment | 11/23/2017 | ST_SOP_002 |
ST-SOP-003 | Inter-Building Shipping SOP | 7/19/2019 | ST_SOP_003 |
Peptide Core Protocols (PC-SOP)
SOP Number | Title | Date | Download Link |
PC-SOP-001 | Peptide Access Procedures | 4/28/2017 | PC-SOP-001 |
PC-SOP-002 | Peptide Weighing | 4/28/2017 | PC-SOP-002 |
PC-SOP-003 | Peptide Storage, Reconstitution and Pools | 5/5/2017 | PC-SOP-003 |
PC-SOP-004 | Peptide Matrix Preparation | 11/23/2017 | PC-SOP-004 |
Processing Lab Protocols (PL)
SOP Number | Title | Date | Download Link |
PL-SOP-001 | DNA Extraction From Ficoll (Gentra Kit) | 4/25/2017 | PL_SOP_001 |
PL-SOP-002 | PBMC _ Plasma Separation From Leukapheresis | 4/25/2017 | PL-SOP-002 |
PL-SOP-003 | DNA Extraction from PBMCs (Qiagen Kit) | 4/25/2017 | PL-SOP-003 |
PL-SOP-004 | Ragon Institute Protocol for Sending DNA for HLA/KIR Typing | 4/25/2017 | PL-SOP-004 |
PL-SOP-005 | Ragon Institute Specimen Distribution Guidelines | 4/27/201717 | PL-SOP-005 |
PL-SOP-006 | DNA Measurement using Nanodrop ND-1000 instrument | 4/27/2017 | PL-SOP-006 |
PL-SOP-007 | PBMC Isolation using Ficoll | 4/27/2017 | PL-SOP-007 |
PL-SOP-008 | Vesphene Use Instructions | 4/27/2017 | PL-SOP-008 |
PL-SOP-009 | Ragon Institute SOP for HLA Shipments | 4/27/2017 | PL-SOP-009 |
PL-SOP-010 | Protocol for Freezing down Cells | 4/27/2017 | PL-SOP-010 |
Tissue Culture (TC-SOP)
SOP Number | Title | Date | Download Link |
TC-SOP-001 | Ragon Institute Tissue Culture Training | 06/06/2017 | TC_SOP_001 |
TC-SOP-002 | EBV Supernatant- B958 growth, cultivation and freezing | 11/23/2017 | TC_SOP_002 |
TC-SOP-003 | Thawing Frozen Cells | 12/25/2017 | TC_SOP_003 |
TC-SOP-004 | CTL Feeding | 11/23/2017 | TC_SOP_004 |
TC-SOP-005 | CTL Cloning | 11/23/2017 | TC_SOP_005 |
TC-SOP-006 | T-Cell Restimulation Procedure | 11/23/2017 | TC_SOP_006 |
TC-SOP-007 | Autoclave Validation Procedure | 11/23/2017 | TC_SOP_007 |
TC-SOP-008 | Guidelines for working with M.bovis BCG in BSL2+ Laboratory | 11/23/2017 | TC_SOP_008 |
TC-SOP-009 | EliSpot Assay | 11/23/2017 | TC_SOP_009 |
TC-SOP-010 | Chromium 52 Release Assay | 11/23/2017 | TC_SOP_010 |
TC-SOP-011 | Intracellular Cytokine Staining | 12/25/2017 | TC_SOP_011 |
TC-SOP-012 | Perkin Elmer p24 ELISA Kit Protocol | 12/25/2017 | TC_SOP_012 |
TC-SOP-013 | Tetramer Staining | 12/25/2017 | TC_SOP_013 |
TC-SOP-014 | PHA Reconstitution | 12/25/2017 | TC_SOP_014 |
TC-SOP-015 | Sterile Media Preparation | 12/25/2017 | TC_SOP_015 |
TC-SOP-016 | Viral Inhibition Assay | 12/25/2017 | TC_SOP_016 |
TC-SOP-017 | Transporting Biosafety Level 2+ (BSL2+) Biohazardous Waste | 11/22/2018 | TC_SOP_017 |
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